This rat IgG2c monoclonal antibody was generated against neoglycoprotein and recognizes land plants heteroxylan.
- Reacts with land plants heteroxylan
- No cross-reactivity with wheat arabinoxylan
- Suitable for Immunofluorescence and ELISA applications
Generated using a neoglycoprotein (xylopentaose-BSA). It can recognise unsubstituted and relatively low-substituted xylans in several species. It has no cross-reactivity with wheat arabinoxylan. Xylan is a group of hemicelluloses that reside in plant cells walls and also can be found in some algae (both green and red). Xylans are polysaccharides whose backbone consists of beta-1,4-linked xylosyl residues. This backbone can be substituted with side-chains of arabinosyl, glucuronosyl, and 4-O-mthylglucuronosyl residues, and can also be further modified by acetyl substitution on the hydroxyls of the xylosyl residues.
|缓冲液/Buffer:||Cell Culture Supernatant, 0.05% Sodium Azide|
|已测试应用/Tested Applications||IF (1:10), ELISA (1:10)|
|存储温度/Storage:||+4C (short term), -20C (long term)|
From the laboratory of Paul Knox, PhD, University of Leeds.
1. McCartney L, Marcus SE, Knox JP. Monoclonal antibodies to plant cell wall xylans and arabinoxylans. J Histochem Cytochem. 2005 Apr;53(4):543-6.
2. Ruprecht C, Bartetzko MP, Senf D, Dallabernardina P, Boos I, Andersen MCF, Kotake T, Knox JP, Hahn MG, Clausen MH, Pfrengle F (2017) A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology https://doi.org/10.1104/pp.17.00737