13C6-UV Cleavable Biotin-Azide
This isotopically labeled “heavy” tag can be used in parallel to the unlabeled “light” analog (See: UV Cleavable Biotin-Azide) to screen protein adduction by identifying hits as those with a mass difference of 6 Da.
Highlights:
* Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
* Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
This isotopically labeled “heavy” tag can be used in parallel to the unlabeled “light” analog (See: UV Cleavable Biotin-Azide) to screen protein adduction by identifying hits as those with a mass difference of 6 Da.
Highlights:
* Biotin conjugate can be subsequently visualized by streptavidin Western techniques and/or enriched using streptavidin purification methods
* Captured target can be released under mild photolysis conditions (365 nm) and results in a significant decrease of background signal due to non-specifically bound proteins
| 产品类型/Product Type: | Small Molecule |
| 产品名称/Name: | 13C6-UV-Biotin-azide |
| 化学式/Components: | C3213C6H51N7O9S |
| 分子量/Molecular Weight: | 787.88 |
| 外观形态/Format: | Pale yellow powder |
| 纯度Purity: | >95% |
| 溶解性/Solubility: | DMSO, MeOH |
| 谱信息/Spectral Information: | 1H, 13C NMR |
| 注意事项/Comments: | Protect from light and hydrolysis conditions |
| 存储温度/Storage: | Upon receipt store at -20C |
| 运输温度/Shipped: | Ambient temperature |
13C6-UV Cleavable Biotin-Azide From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
1. Quantitative Chemoproteomics for Site-Specific Analysis of Protein Alkylation by 4-Hydroxy-2-Nonenal in Cells. Yang, J.; Tallman, K. A.; Porter, N.A.; Liebler, D. C. Analytical Chemistry 2015, 87(5), 2535.
2. A Chemoproteomic Platform to Assess Bioactivation Potential of Drugs. Sun, R.; Shi, F.; Liu, K.; Fu, L.; Tian, C.; Yang, Yong; Tallman, K. A.; Porter, N. A.; Yang, J. Chemical Research in Toxicology 2017, 30(10), 1797.
3. Fu L, Liu K, He J, Tian C, Yu X, Yang J. Direct Proteomic Mapping of Cysteine Persulfidation. Antioxid Redox Signal. 2020 Nov 20;33(15):1061-1076.
4. Meng J, Fu L, Liu K, Tian C, Wu Z, Jung Y, Ferreira RB, Carroll KS, Blackwell TK, Yang J. Global profiling of distinct cysteine redox forms reveals wide-ranging redox regulation in C. elegans. Nat Commun. 2021 Mar 3;12(1):1415.