Biotin-Azide
The Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.
Highlights:
-Biotin-conjugate can be subsequently visualized using streptavidin Western techniques
For enrichment of the alkyne modified substrate, the UV Cleavable Biotin-Azide is recommended.
The Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.
Highlights:
-Biotin-conjugate can be subsequently visualized using streptavidin Western techniques
For enrichment of the alkyne modified substrate, the UV Cleavable Biotin-Azide is recommended.
| 产品类型/Product Type: | Small Molecule |
| 产品名称/Name: | Biotin-Azide |
| 化学式/Components: | C22H39N7O5 |
| 分子量/Molecular Weight: | 513.65 |
| 外观形态/Format: | Solid |
| 溶解性/Solubility: | DMSO, MeOH |
| 来源/Source: | Synthetic |
| 存储温度/Storage: | Upon receipt store at -20C |
| 运输温度/Shipped: | Ambient temperature |
产品说明书：
Biotin-Azide
From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.
1. Assay of Protein and Peptide Adducts of Cholesterol Ozonolysis Products by Hydrophobic and Click Enrichment Methods. Windsor, K.; Genaro-Mattos, T. C.; Miyamoto, S.; Stec, D. F.; Kim, H.-Y. H.; Tallman, K. A.; Porter, N. A. Chem. Res. Toxicol. 2014, 27, 1757.
2. Probing lipid-protein adduction with alkynyl surrogates: application to Smith-Lemli-Opitz syndrome. Windsor, K.; Genaro-Mattos, T. C.; Kim, H.-Y.; Liu, W.; Tallman, K. A.; Miyamoto, S.; Korade Z.; Porter, N. A. J. Lipid Res. 2013, 54, 2842.
3. Identification of Protein Targets of 4-Hydroxynonenal Using Click Chemistry for ex Vivo Biotinylation of Azido and Alkynyl Derivatives. Vila, A.; Tallman, K. A.; Jacobs, A. T.; Liebler, D. C.; Porter, N. A.; Marnett, L. Chem. Res. Toxicol. 2008, 21, 432.
4. Tian C, Sun R, Liu K, Fu L, Liu X, Zhou W, Yang Y, Yang J. Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol. 2017 Nov 16;24(11):1416-1427.e5.
5. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76.
6. Heppner DE, Dustin CM, Liao C, Hristova M, Veith C, Little AC, Ahlers BA, White SL, Deng B, Lam YW, Li J, van der Vliet A. Direct cysteine sulfenylation drives activation of the Src kinase. Nat Commun. 2018 Oct 30;9(1):4522.
7. Dustin CM, Hristova M, Schiffers C, van der Vliet A. Proteomic Methods to Evaluate NOX-Mediated Redox Signaling. Methods Mol Biol. 2019;1982:497-515.