Biotin-Azide

The Biotin-Azide allows the tagging of alkyne modified substrates via copper-catalyzed click chemistry.

Highlights:

-Biotin-conjugate can be subsequently visualized using streptavidin Western techniques

For enrichment of the alkyne modified substrate, the UV Cleavable Biotin-Azide is recommended.

货号 品名 规格 库存 价格
EVU101
Biotin-Azide
10mg 现询
Regular Price:2700元
On Sale:
产品详情

产品类型/Product Type:Small Molecule
产品名称/Name:Biotin-Azide 
化学式/Components:C22H39N7O5 
分子量/Molecular Weight:513.65 
外观形态/Format:Solid
溶解性/Solubility: DMSO, MeOH 
来源/Source: Synthetic
存储温度/Storage: Upon receipt store at -20C 
运输温度/Shipped:Ambient temperature     

文档资料

产品说明书:auto_1317.jpgBiotin-Azide 

产品源头

From the laboratory of Ned A. Porter, PhD and Keri A. Tallman, PhD, Vanderbilt University.

参考文献

1. Assay of Protein and Peptide Adducts of Cholesterol Ozonolysis Products by Hydrophobic and Click Enrichment Methods. Windsor, K.; Genaro-Mattos, T. C.; Miyamoto, S.; Stec, D. F.; Kim, H.-Y. H.; Tallman, K. A.; Porter, N. A. Chem. Res. Toxicol. 2014, 27, 1757.

2. Probing lipid-protein adduction with alkynyl surrogates: application to Smith-Lemli-Opitz syndrome. Windsor, K.; Genaro-Mattos, T. C.; Kim, H.-Y.; Liu, W.; Tallman, K. A.; Miyamoto, S.; Korade Z.; Porter, N. A. J. Lipid Res. 2013, 54, 2842.

3. Identification of Protein Targets of 4-Hydroxynonenal Using Click Chemistry for ex Vivo Biotinylation of Azido and Alkynyl Derivatives.  Vila, A.; Tallman, K. A.; Jacobs, A. T.; Liebler, D. C.; Porter, N. A.; Marnett, L.  Chem. Res. Toxicol. 2008, 21, 432.

4. Tian C, Sun R, Liu K, Fu L, Liu X, Zhou W, Yang Y, Yang J. Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol. 2017 Nov 16;24(11):1416-1427.e5. 

5. Fu L, Liu K, Ferreira RB, Carroll KS, Yang J. Proteome-Wide Analysis of Cysteine S-Sulfenylation Using a Benzothiazine-Based Probe. Curr Protoc Protein Sci. 2019 Feb;95(1):e76. 

6. Heppner DE, Dustin CM, Liao C, Hristova M, Veith C, Little AC, Ahlers BA, White SL, Deng B, Lam YW, Li J, van der Vliet A. Direct cysteine sulfenylation drives activation of the Src kinase. Nat Commun. 2018 Oct 30;9(1):4522. 

7. Dustin CM, Hristova M, Schiffers C, van der Vliet A. Proteomic Methods to Evaluate NOX-Mediated Redox Signaling. Methods Mol Biol. 2019;1982:497-515. 

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